GRP receptor mediates BBS-stimulated PGE
release and COX-2 expression. A) PC-3 cells were pre-treated with BIM26226 (1 μM) for 30 min and then incubated with or without 10 nM of BBS over a time course. The amount of PGE2 released into the culture media was assessed by enzyme-linked immunoassay (Methods). The data was normalized to total cellular protein per well. [† BBS vs. vehicle (no treatment), p < 0.01; * BBS+BIM26226 vs. BBS alone, p < 0.01]. B) Immunoblot showing the effects of BIM26226 on BBS-stimulated COX-2 protein expression. PC-3 cells were pretreated with BIM26226 for 30 min and then treated with BBS for 4 h. Blots were reprobed for β-actin to ensure the equivalent loading and transfer of protein samples.