BBS treatment induces a time- and dose-dependent increase in COX-2 mRNA and protein expression. A) Autoradiography of Northern blots demonstrating time-dependent increase in COX-2 mRNA steady-state levels in PC-3 cells treated with 10 nM of BBS. Blots were reprobed for 18S ribosomal RNA to ensure equivalent loading and transfer of total RNA in each lane. B) Immunoblot showing time-dependent increase in COX-2 protein levels following treatment with BBS (10 nM). Blots were reprobed for β-actin to ensure the equivalent loading and transfer of protein samples. C, D) Effects of a 4-h treatment with different concentrations of BBS on COX-2 mRNA (C) and protein (D) expression.