|
Cells
|
siRNA
|
G1/G0 (% of total)
|
S (% of total)
|
G2/M (% of total)
|
|---|
|
U-2987 MG
|
Control
|
84.58 ± 0.16
|
9.95 ± 0.18
|
5.44 ± 0.1
|
| |
CGGBP1
|
89.68 ± 0.28
|
2.85 ± 0.04
|
7.45 ± 0.29
|
|
U-2987 MG-vector
|
Control
|
64.59 ± 0.72
|
26.83 ± 0.85
|
8.57 ± 0.36
|
| |
UTR
|
70.03 ± 0.18
|
22.34 ± 0.35
|
7.51 ± 0.12
|
|
U-2987 MG-CGGBP1
|
Control
|
60.82 ± 2.48
|
28.56 ± 3.04
|
10.61 ± 0.71
|
| |
UTR
|
60.52 ± 2.14
|
28.46 ± 3.56
|
11.01 ± 1.49
|
|
HCT116 p53+/+;p21+/+
|
Control
|
87.12 ± 0.39
|
11.39 ± 0.05
|
1.48 ± 0.38
|
| |
CGGBP1
|
91.94 ± 0.64
|
6.88 ± 1.16
|
1.17 ± 0.51
|
|
HCT116 p53-/-;p21+/+
|
Control
|
87.46 ± 0.47
|
10.15 ± 0.44
|
2.38 ± 0.02
|
| |
CGGBP1
|
91.54 ± 0.05
|
7.66 ± 0.03
|
0.8 ± 0.04
|
|
HCT116 p53+/+; p21-/-
|
Control
|
88.44 ± 0.45
|
10.50 ± 0.39
|
1.05 ± 0.06
|
| |
CGGBP1
|
92.76 ± 0.22
|
5.55 ± 0.24
|
1.67 ± 0.02
|
|
U2OS
|
Control
|
49.82 ± 0.35
|
37.09 ± 0.45
|
13.08 ± 0.55
|
| |
CGGBP1
|
68.83 ± 0.58
|
19.29 ± 0.25
|
11.86 ± 0.47
|
|
SAOS2
|
Control
|
66.34 ± 0.73
|
20.58 ± 0.57
|
12.65 ± 0.50
|
| |
CGGBP1
|
75.65 ± 0.49
|
11.88 ± 0.25
|
12.46 ± 0.52
|
|
WT (Ink4a+/+;Arf+/+)
|
Control
|
66.97 ± 0.82
|
29.85 ± 0.91
|
3.14 ± 0.12
|
| |
CGGBP1
|
75.42 ± 0.58
|
20.79 ± 0.76
|
3.78 ± 0.19
|
|
Ink4a-/-
|
Control
|
71.52 ± 0.61
|
27.18 ± 0.16
|
1.29 ± 0.64
|
| |
CGGBP1
|
77.91 ± 0.64
|
17.82 ± 0.36
|
4.26 ± 0.32
|
|
Arf-/-
|
Control
|
56.33 ± 1.07
|
40.13 ± 1.32
|
3.53 ± 0.62
|
| |
CGGBP1
|
68.99 ± 0.78
|
28.80 ± 0.74
|
2.19 ± 0.05
|
|
U-2987 MG
|
Control
|
76.04 ± 0.49
|
18.75 ± 5.21
|
5.21 ± 0.32
|
| |
CGGBP1
|
84.88 ± 0.60
|
8.04 ± 7.07
|
7.07 ± 0.42
|
| |
GAS1
|
72.33 ± 0.40
|
20.22 ± 0.20
|
7.44 ± 0.60
|
| |
CGGBP1+ GAS1
|
92.44 ± 0.32
|
3.79 ± 0.08
|
3.76 ± 0.23
|
- N = 3 independent transfections in each case. The differences between the G1/G0 phase and S phase values for control siRNA and CGGBP1 siRNA (or between control siRNA and CGGBP1+GAS1 siRNA combined for the last sample) are significant (Heteroscedastic two tailed T test, p < 0.05). Difference between control siRNA and UTR siRNA is significant for U-2987 MG-vector But insignificant for U-2987 MG-CGGBP1 (Heteroscedastic two tailed T test, p < 0.05). Please note that close to 50% reductions in the S phase populations of most samples due to CGGBP1 knockdown is reflected as a rather minor yet significant increase in the G0/G1 phase populations.
