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Table 1 Effect of CGGBP1 depletion in different cell types on the percentage of cells in different stages of cell cycle as measured by flow cytometric analysis of DNA content per nucleus

From: CGGBP1 regulates cell cycle in cancer cells

Cells siRNA G1/G0 (% of total) S (% of total) G2/M (% of total)
U-2987 MG Control 84.58 ± 0.16 9.95 ± 0.18 5.44 ± 0.1
  CGGBP1 89.68 ± 0.28 2.85 ± 0.04 7.45 ± 0.29
U-2987 MG-vector Control 64.59 ± 0.72 26.83 ± 0.85 8.57 ± 0.36
  UTR 70.03 ± 0.18 22.34 ± 0.35 7.51 ± 0.12
U-2987 MG-CGGBP1 Control 60.82 ± 2.48 28.56 ± 3.04 10.61 ± 0.71
  UTR 60.52 ± 2.14 28.46 ± 3.56 11.01 ± 1.49
HCT116 p53+/+;p21+/+ Control 87.12 ± 0.39 11.39 ± 0.05 1.48 ± 0.38
  CGGBP1 91.94 ± 0.64 6.88 ± 1.16 1.17 ± 0.51
HCT116 p53-/-;p21+/+ Control 87.46 ± 0.47 10.15 ± 0.44 2.38 ± 0.02
  CGGBP1 91.54 ± 0.05 7.66 ± 0.03 0.8 ± 0.04
HCT116 p53+/+; p21-/- Control 88.44 ± 0.45 10.50 ± 0.39 1.05 ± 0.06
  CGGBP1 92.76 ± 0.22 5.55 ± 0.24 1.67 ± 0.02
U2OS Control 49.82 ± 0.35 37.09 ± 0.45 13.08 ± 0.55
  CGGBP1 68.83 ± 0.58 19.29 ± 0.25 11.86 ± 0.47
SAOS2 Control 66.34 ± 0.73 20.58 ± 0.57 12.65 ± 0.50
  CGGBP1 75.65 ± 0.49 11.88 ± 0.25 12.46 ± 0.52
WT (Ink4a+/+;Arf+/+) Control 66.97 ± 0.82 29.85 ± 0.91 3.14 ± 0.12
  CGGBP1 75.42 ± 0.58 20.79 ± 0.76 3.78 ± 0.19
Ink4a-/- Control 71.52 ± 0.61 27.18 ± 0.16 1.29 ± 0.64
  CGGBP1 77.91 ± 0.64 17.82 ± 0.36 4.26 ± 0.32
Arf-/- Control 56.33 ± 1.07 40.13 ± 1.32 3.53 ± 0.62
  CGGBP1 68.99 ± 0.78 28.80 ± 0.74 2.19 ± 0.05
U-2987 MG Control 76.04 ± 0.49 18.75 ± 5.21 5.21 ± 0.32
  CGGBP1 84.88 ± 0.60 8.04 ± 7.07 7.07 ± 0.42
  GAS1 72.33 ± 0.40 20.22 ± 0.20 7.44 ± 0.60
  CGGBP1+ GAS1 92.44 ± 0.32 3.79 ± 0.08 3.76 ± 0.23
  1. N = 3 independent transfections in each case. The differences between the G1/G0 phase and S phase values for control siRNA and CGGBP1 siRNA (or between control siRNA and CGGBP1+GAS1 siRNA combined for the last sample) are significant (Heteroscedastic two tailed T test, p < 0.05). Difference between control siRNA and UTR siRNA is significant for U-2987 MG-vector But insignificant for U-2987 MG-CGGBP1 (Heteroscedastic two tailed T test, p < 0.05). Please note that close to 50% reductions in the S phase populations of most samples due to CGGBP1 knockdown is reflected as a rather minor yet significant increase in the G0/G1 phase populations.