CGGBP1 regulates H3K9 trimethylation levels at CDKN1A and GAS1 promoters. A: CGGBP1 binds to CDKN1A promoter and this is sensitive to CGGBP1 siRNA, thus showing the specificity of the assay. B: H3K9 trimethylation is reduced at the CDKN1A promoter after CGGBP1 depletion whereas as shown in C, H3K16 acetylation was not significantly changed. D: CGGBP1 binds to GAS1 promoter in a CGGBP1 siRNA-sensitive manner. E: H3K9 trimethylation in GAS1 promoter region is reduced by CGGBP1 depletion whereas the H3K16 acetylation in this region was unaffected by CGGBP1 depletion (F). For exact region details of the assayed region of CDKN1A and GAS1 promoters, see primers mentioned in materials and methods. ChIP DNA samples from 3 independent assays were pooled and analyzed in triplicates. Input DNA was used to normalize the amount of DNA and used as baseline in double delta Ct calculations to find out relative changes in DNA-protein interactions. Changes in real-time PCR are significant (p < 0.05, heteroscedastic two-tailed T-test) in B and E only. For B, C, E and F, the black bars represent the control siRNA treated samples and the white bars represent CGGBP1 siRNA treated samples.