Figure 5

Lack of the HSE-HSF interaction in LA treated B16F10 cells. Nuclear extracts were prepared from B16F10 cells maintained at 37°C (lane 1), incubated at 42,5°C for 0,5h (2) and 1h (3) or treated with Lipofectin (Gibco) followed by recovery: 30 min (4), 1h (5), 2h (6). CHBA - constitutive HSE-binding activity corresponding to a Ku protein-containing complex; F - free HSE oligonucleotide.