Figure 2

Comparison of activation of Hspa1b promoter by different transfection agents (CAT assay). The B16F10 cells were transfected with p950/CAT6 construct containing Hspa1b promoter linked with CAT reporter gene using: Lipofectin (Gibco), DEAE-Dextran (Pharmacia), DDAB/DOPE (home made) and Lipofectamine 2000 (Invitrogen). K - cells grown at 37°C for 6 or 48 h after transfection. HS - heat-shocked cells, grown at 37°C for 24 h after transfection, then heat shocked at 42,5°C for 45 min followed by recovery for 24 h at 37°C. AcCM - acetylchloramphenicol, product of the enzymatic assay. CM - non-acetylated chloramphenicol, substrate of the assay.