Immunoblotting with domain-specific anti-peptide antibodies. Each MD antibody shares the color with the recognized domains: MD2, Longin domain, red; MD3, SNARE motif, cyan; MD4 and MD5, unknown function region of VAMP7b, magenta. Other represented regions are the N-terminal region of VAMP7c (grey) and the transmembrane region with intravesicular tail (light green). The two large blots share the same series of whole lysates from E. coli cells (lanes 1-5) or Jurkat cells (lane 6) and each blot was incubated with antibodies indicated on the top. Bacterial cells express empty control plasmids (lane 1, pGEX; lane 3, pET32H), or the following VAMP7 fragments: cytoplasmic of VAMP7a fused C-terminally to GST in pGEX (lane 2), LD of VAMP7a fused C-terminally to thioredoxin in pET32H (lane 4), C-ter region of VAMP7b fused C-terminally to thioredoxin in pET32H (lane 5). Molecular weight markers are reported.