Figure 4

Nuclear localization of pXbp1 and the ERAI vector system. (A) pXbp1 was immunostained with the Xbp1 antibody in the TM treated PEF cells. pXbp1 protein was localized in cytoplasm under the normal condition but was predominantly translocated into the nuclei by the ER stress. (B) Detection of the ER stress-activated indicator (ERAI) by the green fluorescence (green) in the TM treated PEF cells (arrows). DAPI is used for marking the nuclei. (C) In the Western blot analysis with the ant-FLAG antibody, FLAG-tagged XBP1-venus fusion protein was found in PEF cells transfected with ERAI plasmids and treated with TM. The upper bands indicate the XBP1-venus fusion protein (45 kDa). The lower bands indicate the truncated XBP1 (30 kDa).