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Table 1 RmInt1 splicing activity in S.meliloti measured by relative quantification of joined exons in qRT-PCR

From: Exon sequence requirements for excision in vivo of the bacterial group II intron RmInt1

Construction

% splicing ± errora

WT

100

DV*

0.76 ± 0.01

+1A

98 ± 1

  1. aRmInt1 splicing is calculated by 2-ΔΔCTMethod (21) using qRT-PCR primers and conditions specified in Methods section.