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Table 1 The primers used in this study

From: GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity

Abbreviation

Sequence (5'--3')

Description

MP1

AARGGNAGYTAYGGNGTNGT

Degenerate primer, forward

MP2

GCNACRTARTCNGTCCARAA(H = A, C, or T; N = A, C, G, or T; R = A or G; V = A, C, or G; Y = C or T)

Degenerate primer, reverse

3P1

TTCTTTATCAGCTTCTTCGGG

3' RACE reverse primer, outer

3P2

TTCATCGGGATCTAAAGCCG

3' RACE reverse primer, nested

B26

GACTCGAGTCGACATCGAT (T)18

Universal primer, outer

B25

GACTCGAGTCGACATCGAT

Universal primer, nested

5P1

GAAGGAGGGAACAATATGTGC

5' RACE reverse primer, outer

5P2

ATGACGTAGGAGCCTGAGAAG

5' RACE reverse primer, nested

AAP

GGCCACGCGTCGACTAGTAC (G)14

Abridged anchor primer

AUAP

GGCCACGCGTCGACTAGTAC

Abridged universal amplification primer

FL1

GATGCTAAGATGCAGCCTGATC

Full-length cDNA primer

FL2

GCTTCACTTGTAACTTGTCTGAGC

Full-length cDNA primer

Z1

CCGCACTTGGATACATGAAGCC

Genomic sequence primer

Z2

GAGTATCTGGAAGGATCAGAGCC

Genomic sequence primer

5P3

GCTGCCAGACCTGGGAAAGTAG

primer used for probe synthesized

Wup1

CACGCCATTAAACCAAGTAG

I-PCR primer

Wdra1

GAGGTTGACTAACACTAGTG

I-PCR primer

Wup2

GATCAGGCTGCATCTTAGCATC

I-PCR primer

Wdra2

AGCGTATGACACGCATACTG

I-PCR primer

Wbd-1

GCGC TCTAGA GGGGCTTCCTGTTTGATGCC Xba I

Coding region primer

Wbd-2

GCGC GTCGAC CCTCAACCAGCAGTAGGAAG Sal I

Coding region primer

W-GFP

GCGC CTCGAG ATACCACTGACTTGATCCAG Xho I

Coding region primer

AtEF-1α-F

ATGGGTAAAGAGAAGTTTCACAT

RT-PCR primer

AtEF-1α-R

CTTGTTACAACAGCAGATCAT

RT-PCR primer

AtPR1-F

GACGAGAGGGTTGCAGCCTATG

RT-PCR primer

AtPR1-R

GATTCTCGTAATCTCAGCTCT

RT-PCR primer

AtPR4-F

CCACCTACCATTTCTATAATCC

RT-PCR primer

AtPR4-R

CACAGTCGAGAAATTGGTAGTC

RT-PCR primer

AtPDF1.2-F

TCATGGCTAAGTTTGCTTCC

RT-PCR primer

AtPDF1.2-R

AATACACACGATTTAGCACC

RT-PCR primer