Subcellular localization of the GhMPK16 protein in onion epidermal cells. (A) Schematic diagram of the 35S-GhMPK16::GFP and 35S-GFP fusion construct. (B) Transient expression of 35S-GFP and 35S-GhMPK16::GFP in onion epidermal cells. Cells were analyzed by laser confocal microscopy 12 h after particle bombardment. The nuclei of the onion cells were visualised by DAPI staining. Bar = 20 μm.