Figure 3
From: The four Zn fingers of MBNL1 provide a flexible platform for recognition of its RNA binding elements
Analysis of RNA spacing between GC dinucleotides on MBNL1 binding. A) RNAs used in analysis of GC spacing. Name of RNA indicates the number of nucleotides between the two GC dinucleotides. GC dinucleotides are in bold. Next to each RNA is the corresponding Kd, averaged over at least three independent experiments. B) Representative results from the gel shift assays. The RNA being tested is to the right of each gel and at the top of the column is the concentration of protein (nM) in each lane.