Figure 2
From: The four Zn fingers of MBNL1 provide a flexible platform for recognition of its RNA binding elements
Determination of the importance of nucleotides adjacent to the GC dinucleotides for MBNL1 binding. A) RNAs used to analyze the sequence outside of the GC dinucleotides. Nucleotides that have been changed from the 2GC RNA are in bold. Next to the RNAs are the corresponding Kds, averaged over at least three independent experiments. B and C) Representative gel shifts assays. MBNL1 concentrations are at the top of each column. RNA being tested is on the left side of the gels.