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Table 1 Selection of candidate reference genes from microarray data.

From: Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Gene symbol

Definition

Average intensity

Primary multi-fun term

ssrA

tmRNA

13.88

cell processes -> protection -> drug resistance/sensitivity

rnpB

RnpB RNA; catalytic subunit of RNAse P

13.42

information transfer -> RNA related -> RNA degradation

pflC

probable pyruvate formate lyase 2 activating enzyme

8.650

information transfer -> protein related -> posttranslational modification

hycG

hydrogenase 3 and formate hydrogenlyase complex

8.09

metabolism -> energy metabolism, carbon -> anaerobic respiration

uxuB

D-mannonate oxidoreductase

8.94

metabolism -> carbon utilization -> carbon compounds

ygjD

YgjD, target for YeaZ protease

9.74

regulation -> type of regulation -> posttranscriptional -> proteases, cleavage of compounds

uxuR

UxuR-fructuronate

8.53

information transfer -> RNA related -> Transcription related

yajR

YajR MFS transporter

8.54

cell processes -> protection -> drug resistance/sensitivity

asnA

asparagine synthetase A

8.78

metabolism -> biosynthesis of building blocks -> amino acids -> asparagine

hcaT

HcaT MFS transporter

8.292

cell structure -> membrane

ldnT

L-idonate/5-ketogluconate/gluconate transporter

8.991

cell structure -> membrane

yghB

conserved inner membrane protein

9.01

cell structure -> membrane

ugpQ

glycerophosphodiester phosphodiesterase, cytosolic

9.16

metabolism -> central intermediary metabolism -> misc. glycerol metabolism

metL

aspartate kinase/homoserine dehydrogenase

8.973

metabolism -> biosynthesis of building blocks -> amino acids -> homoserine

pbpC

putative peptidoglycan enzyme

8.73

cell structure -> murein

ilvY

IlvY DNA binding transcriptional dual regulator

9.320

information transfer -> RNA related -> Transcription related

phnN

ribose 1,5-bisphosphokinase

8.05

metabolism -> metabolism of other compounds -> phosphorous metabolism

cysG

uroporphyrin III C-methyltransferase [multifunctional]

8.219

metabolism -> biosynthesis of building blocks -> cofactors, small molecule carriers -> heme, porphyrine

cca

tRNA nucleotidyltransferase

9.19

information transfer -> RNA related -> RNA modification

rrsA

rrsA 16S ribosomal RNA

14.6

cell structure -> ribosomes

ihfB

integration host factor β subunit

  
  1. Meta-analysis of the expression profiles of 240 arrays relevant to recombinant protein production. Twenty candidate reference genes were selected based on the lowest CV. The log2 transformed values of the average signal intensities among the 240 arrays were shown as Mean. ihfB was included for comparison, and commonly used reference genes were highlighted.