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Figure 5 | BMC Molecular Biology

Figure 5

From: Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Figure 5

Fold changes in crtE expressions normalized using different reference gene(s) in E. coli DH10B. Fold changes in transcript expressions of crtE in IPTG induced cells relative to that of control were normalized by (1) geometric mean of top three most stable genes in E. Coli DH10B - cysG/rrsA/pbpC; (2) geometric mean of top three most stable genes in E. Coli BL21 - idnT, cysG, and hcaT; (3) rrsA or (4) ihfB. 2 is the threshold for upregulation and 0.5 is the threshold for downregulation.

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BMC Molecular Biology

ISSN: 1471-2199

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