Figure 3

Comparison of the normalization factors calculated using different reference gene(s) in E. Coli BL21 (DE3). Normalization factors (NFs) calculated with idnT/cysG/hcaT, rrsA and ihfB were compared to that calculated by the top 3 reference genes (NFtop3) as recommended by both NormFinder and geNorm, for each stimulus. The percentage deviations of NFldnT/cysG/hcaT; NFrrsA; NFihfB from NFtop3 (|NFx-NFtop3|/NFtop3) were represented by box plot. The 25th percentile to the 75th percentile (boxes), and ranges (whiskers) were shown.