Figure 3From: Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCRComparison of the normalization factors calculated using different reference gene(s) in E. Coli BL21 (DE3). Normalization factors (NFs) calculated with idnT/cysG/hcaT, rrsA and ihfB were compared to that calculated by the top 3 reference genes (NFtop3) as recommended by both NormFinder and geNorm, for each stimulus. The percentage deviations of NFldnT/cysG/hcaT; NFrrsA; NFihfB from NFtop3 (|NFx-NFtop3|/NFtop3) were represented by box plot. The 25th percentile to the 75th percentile (boxes), and ranges (whiskers) were shown.Back to article page