Table 5 Effect of temperature on the rate of ssDNA-dependent ATP and dATP hydrolysis catalyzed by Dge RecA and Dmu RecA.
Protein | Substrate | Rate of hydrolysis (μM/min) | ||||
|---|---|---|---|---|---|---|
32°C | 37°C | 42°C | 45°C | 50°C | ||
Dge RecA | ATP | 19.8 ± 0.26 | 26.8 ± 0.40 | 31.2 ± 0.56 | 34.1 ± 0.78 | 26.6 ± 0.43 |
dATP | 10.3 ± 0.18 | 31.6 ± 0.63 | 53.7 ± 1.18 | 65.7 ± 1.71 | 56.5 ± 1.30 | |
Dmu RecA | ATP | 15.6 ± 0.23 | 34.4 ± 0.58 | 55.2 ± 1.16 | 47.6 ± 1.20 | 34.5 ± 0.62 |
dATP | 24.2 ± 0.34 | 47.0 ± 0.71 | 70.3 ± 1.76 | 59.9 ± 1.38 | 43.9 ± 0.88 | |