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Table 3 Effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by Dge RecA.

From: RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation

Substrate

Rate of hydrolysis (μM/min)

 

absence of DNA

dsDNA

ssDNA

ssDNA and dsDNAa

ATP

not detected

not detected

26.8 ± 0.40

20.3 ± 0.36

dATP

not detected

not detected

31.6 ± 0.63

23.7 ± 0.43

  1. Reactions conditions: 37°C, 2.27 mM ATP or dATP.
  2. a DNA strand exchange conditions
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BMC Molecular Biology

ISSN: 1471-2199

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