Table 1 Summary of the purification of Dge RecA and Dmu RecA proteins obtained from 1 L of E. col i BLR(DE3) culture.
Protein | Purification step | Total protein (mg) | Recovery of protein (%) |
|---|---|---|---|
Dge RecA | Cell extract | 760 | 100 |
Heat treatment | 341 | 45 | |
Fractogel EMD | 121 | 16 | |
DEAE column | |||
ResourceQ column | 75 | 10 | |
MonoQ column | 63 | 8 | |
Dmu RecA | Cell extract | 510 | 100 |
Fractogel EMD | 356 | 70 | |
DEAE column | |||
ResourceQ column | 93 | 18 | |
MonoQ column | 50 | 10 |