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Figure 2 | BMC Molecular Biology

Figure 2

From: RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation

Figure 2

SDS-PAGE analysis of the fractions obtained by expression and purification of Dge RecA and Dmu RecA proteins. Lane 1 - LMW SDS Marker (Amersham Biosciences AB): 97, 66, 45, 30, 20.1 and 14.4 kDa, lane 2 - cell extract of the E. coli BLR(DE3) + pET-30Ek/LIC-Dge RecA, lane 3 - Dge RecA after heat treatment, lane 4 - Dge RecA after chromatography on Fractogel EMD DEAE column, lane 5 - Dge RecA after chromatography on ResourceQ column, lane 6 - Dge RecA after chromatography on MonoQ column, lane 7 - Unstained Protein Molecular Weight Marker (Fermentas): 116, 66.2, 45, 35, 25, 18.4 and 14.4 kDa, lane 8 - cell extract of E. coli BLR(DE3) + pET-30Ek/LIC-Dmu RecA, lane 9 - Dmu RecA after chromatography on Fractogel EMD DEAE column, lane 10 - Dmu RecA after chromatography on ResourceQ column, lane 11 - Dmu RecA after chromatography on MonoQ column.

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BMC Molecular Biology

ISSN: 1471-2199

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