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Figure 1 | BMC Molecular Biology

Figure 1

From: Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae

Figure 1

Purification of SXT-Exo and lambda-Exo, and determination of their multimericity by size exclusion chromatography. Panel A: Size exclusion chromatogram of purified SXT-Exo protein expressed from plasmid pEA1-1. Panel B: Size exclusion chromatogram of purified lambda-Exo protein expressed from plasmid pEE4. Panel C: 12% polyacrylamide gel (SDS-PAGE) analysis of the SXT-Exo purification procedure and purified SXT-Bet, SXT-Ssb, lambda-Bet and lambda-Exo proteins; lane 1: Benchmark protein ladder (Invitrogen); lane 2: pEA1-1/E. coli BL21 (DE3) pLysS Rosetta whole cell extract immediately prior to induction; lane 3: whole cell extract 6 hours after induction with IPTG; lane 4: supernatant from cell extract 6 hours post induction; lane 5: purified SXT-Exo; lane 6: purified SXT-Bet expressed from pX28-1; lane 7: purified SXT-Ssb expressed from pSB2; lane 8: purified lambda-Bet expressed from p1DB; lane 9: purified lambda-Exo expressed from pEE4.

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BMC Molecular Biology

ISSN: 1471-2199

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