Table 2 Primer pairs for reverse-transcriptase quantitative PCR and RACE-PCR

Reverse-transcriptase quantitative PCR

CSN2

F: 5'-GAGGATGACCTGAAGAAGGGAACA-3'

145

97.1%

R: 5'-TAAGTGGATGGGGAATTGCCG-3'

CSN4

F: 5'-GCAGGGCAGCAGCGTTCAAGA-3'

111

96.2%

R: 5'-ACGGATGATGCGGTCCAGGTG-3'

CSN5

F: 5'-CGAGGGAACGGAGACAAGAGT-3'

148

97.5%

R: 5'-TCCAGACAGCCAGCAACCATA-3'

CSN6

F: 5'-CAGTTTCTCTTCACCCTCTGGTCATA-3'

115

97.6%

R: 5'-GTTTGTTTTCCAATCAAGGCTCC-3'

CSN7A

F: 5'-CTCTTTGCTTATGGTGTTTATGCTGA-3'

128

99.8%

R: 5'-ATACTCTTCTGCCCCGTTGCC-3'

CSN8

F: 5'-TGGTTGCTCGGGCATATACATC-3'

128

99.0%

R: 5'-AATCATTCTCGTGGGTTCGTCAT-3'

SUMO-1

F: 5'-AGAAGGGGAAGGGAACGAATACA-3'

148

98.2%

R: 5'-ACGCAGCGATGCTACAGGGA-3'

Zipper

F: 5'-TGTGAGACAGACCTGAACGACCTT-3'

122

95.4%

R: 5'-ATCTACACGCATCATTGCTTGGGC-3'

EF-1α

F: 5'-TTCCGACTCCAAGAACGACC-3'

122

96.5%

R: 5'-GAGCAGTGTGGCAATCAAGC-3'

RACE-PCR

CSN5

3' RACE-PCR

F: 5'-GACCTACCCAAAGGGATACAAGCCACCG-3'

CSN6

5' RACE-PCR

R: 5'-TGGGCTAACATCACTGAGGGCACTACCG-3'

3' RACE-PCR

F: 5'-GTCAGCGAGGCGAGGTGGAGTTCAAT-3'