Figure 4

Gene expression analysis by the UniShrimpChip comparing P. monodon transcript levels in testis of wild broodstock from the West and East coasts of Thailand (WBW and WBE, respectively), domesticated broodstock at 18, 14, and 10 months (DB-18, DB-14, and DB-10, respectively) using testes pooled from 114 juveniles (JN) as a common reference. Testis from broodstock was labeled with Cy5 fluorescent dye (red) and pooled testes from juveniles with Cy3 fluorescent dye (green). Hierarchical clustering analysis of the 1,439 transcripts present in at least 14 of the 18 microarrays and whose expression differences were greater than 1.5 fold in at least 9 of the 18 microarrays. Clusters 1-4 show examples of various patterns of differentially expressed transcripts. Transcripts in blue letters were further characterized by quantitative real-time PCR.