From: Mapping and functional characterization of the murine Smoothelin-like 1 promoter
RT-PCR & nested PCR | |
---|---|
mSmtnl1-F1 | AAGAAGGATCGAGCACCAGAAC |
mSmtnl1-R1 | ACACACTTGGAATCGGGCAC |
mSmtnl1-nested-F1 | GGAAGGCCATCATGGACAAATTT |
mSmtnl1-nested-R1 | GCACAGTCGGCTAGTTTCTCTG |
5'-RACE | |
5'-CDS | dT25VN (N = A, C, G, or T; V = A, G, or C) |
SMART II A | AAGCAGTGGTATCAACGCAGAGTACGCGGG |
UPM | 5:1 mixture of (1): (2) (1) CTAATACGACTCACTATAGCAAGCAGTGGTATCAACGCAGAGT (2) CTAATACGACTCACTATAGC |
NUPM | AAGCAGTGGTATCAACGCAGAGT |
GSP1 | CCACCTCCAGCAGCTGGGCACAGTCGGCTAG |
GSP2 | GCCTCGTTCTAGCCGAGCTGCCTCACTCTCCAT |
GSP3 | CACCCCACCTTCAAGAGGCCCGGGCTTCG |
NGSP1 | CACCCCACCTTCAAGAGGC |
NGSP2 | CCAACTCCTTTGTGTCACTTCCG |
RPA | |
RPA2 | TAATACGACTCACTATAGGGCTGATCAGATGCTAGGTACACTT GCTTCCACCACCCCTTGCTTCCACC |
T7 promoter | TAATACGACTCACTATAGGG |
RPA-NCS | GAGAGGGACGCAGCAGCGTG |
RPA-NCA | GGTTCTGCCTGCAGCTCGG |
RPA-M1-2S | TAGAACGAGGCAAGAGCTCC |
RPA-M1-2A | GTTCTGCCCAATCTGAAGGC |
RPA-FR1 | GCCACCTGTCAGATCTTCGG |
RPA-RT7 | TAATACGACTCACTATAGGGCTGATCAGATGCTAGGTACAG TTCTGCCCAATCTGAAG |
Promoter Truncations | |
+100 bp LUC R1 | GTGGGATCCGTGAAAGGTAAGAATAGG |
0 bp LUC F1 | CTAGCCTTCTAGAACGAGGCAAGAGCTCC |
-118 bp LUC F2 | CTAGAATTCCTTCCCGAAGCCCGGGC |
-218 bp LUC F3 | CTCGAATTCGCAGCGTGATTGAAAGATG |
-468 bp LUC F4 | CTAGAATTCCCCCTCAGCAGCTACTTTG |
-767 bp LUC F5 | CTCGAATTCCGTTGGGAACTGGGAATTCCG |
-1350 bp LUC F6 | CTAGAATTCGACAAAGCTTGGGGTCTATATC |
-1637 bp LUC F7 | CTAGAATTCCTCTTAACCTCCAAGACATC |
-1869 bp LUC F8 | CTAGAATTCCTCTTAACCTCCAAGACATC |
-2368 bp LUC F9 | CTAGAATTCCAAAGTCCTATTGCTTGCACC |
-2759 bp LUC F10 | GCTGAATTCGAATTATCTCATGGACTTGCCTAGAG |