Skip to main content

Table 1 PCR primers used in the analysis of Smtnl1.

From: Mapping and functional characterization of the murine Smoothelin-like 1 promoter

RT-PCR & nested PCR

 

mSmtnl1-F1

AAGAAGGATCGAGCACCAGAAC

mSmtnl1-R1

ACACACTTGGAATCGGGCAC

mSmtnl1-nested-F1

GGAAGGCCATCATGGACAAATTT

mSmtnl1-nested-R1

GCACAGTCGGCTAGTTTCTCTG

5'-RACE

 

5'-CDS

dT25VN (N = A, C, G, or T; V = A, G, or C)

SMART II A

AAGCAGTGGTATCAACGCAGAGTACGCGGG

UPM

5:1 mixture of (1): (2)

(1) CTAATACGACTCACTATAGCAAGCAGTGGTATCAACGCAGAGT

(2) CTAATACGACTCACTATAGC

NUPM

AAGCAGTGGTATCAACGCAGAGT

GSP1

CCACCTCCAGCAGCTGGGCACAGTCGGCTAG

GSP2

GCCTCGTTCTAGCCGAGCTGCCTCACTCTCCAT

GSP3

CACCCCACCTTCAAGAGGCCCGGGCTTCG

NGSP1

CACCCCACCTTCAAGAGGC

NGSP2

CCAACTCCTTTGTGTCACTTCCG

RPA

 

RPA2

TAATACGACTCACTATAGGGCTGATCAGATGCTAGGTACACTT

GCTTCCACCACCCCTTGCTTCCACC

T7 promoter

TAATACGACTCACTATAGGG

RPA-NCS

GAGAGGGACGCAGCAGCGTG

RPA-NCA

GGTTCTGCCTGCAGCTCGG

RPA-M1-2S

TAGAACGAGGCAAGAGCTCC

RPA-M1-2A

GTTCTGCCCAATCTGAAGGC

RPA-FR1

GCCACCTGTCAGATCTTCGG

RPA-RT7

TAATACGACTCACTATAGGGCTGATCAGATGCTAGGTACAG

TTCTGCCCAATCTGAAG

Promoter Truncations

 

+100 bp LUC R1

GTGGGATCCGTGAAAGGTAAGAATAGG

0 bp LUC F1

CTAGCCTTCTAGAACGAGGCAAGAGCTCC

-118 bp LUC F2

CTAGAATTCCTTCCCGAAGCCCGGGC

-218 bp LUC F3

CTCGAATTCGCAGCGTGATTGAAAGATG

-468 bp LUC F4

CTAGAATTCCCCCTCAGCAGCTACTTTG

-767 bp LUC F5

CTCGAATTCCGTTGGGAACTGGGAATTCCG

-1350 bp LUC F6

CTAGAATTCGACAAAGCTTGGGGTCTATATC

-1637 bp LUC F7

CTAGAATTCCTCTTAACCTCCAAGACATC

-1869 bp LUC F8

CTAGAATTCCTCTTAACCTCCAAGACATC

-2368 bp LUC F9

CTAGAATTCCAAAGTCCTATTGCTTGCACC

-2759 bp LUC F10

GCTGAATTCGAATTATCTCATGGACTTGCCTAGAG