Figure 7
From: TEAD1-dependent expression of the FoxO3a gene in mouse skeletal muscle
Knock down of TEAD1 expression using siRNA. (A) C2C12 cells were treated with siRNA against TEAD1 (SiTEAD1) or negative control (SiControl) or lipofectamine 2000 control (LipoControl) and western blot confirmed the suppression of TEAD1 in SiTEAD1 treated cells. (B) qRT-PCR analysis of FoxO3a using RNA extracted from SiTEAD1 or SiControl or LipoControl. FoxO3a expression was down-regulated about 50% compared to β-actin.