Figure 5

Transient-transfection assays of FoxO3a promoter-luciferase reporters. (A) A transient-transfection experiment in C2C12 cells was performed with a segment of FoxO3a cloned in either the forward or reverse upstream orientation of luciferase. The y axis of the graph represents the relative luciferase activity, with the transfected material shown on the x axis. The pGL3-Basic represents the luciferase vector lacking a promoter. (B) A transient-transfection assay was performed with the FoxO3a promoter-luciferase reporter transfected into C2C12 cells in the presence of a TEAD1 expression vector or the pCDNA3.1 vector as a control. The statistical significance of the differences between the FoxO3a forward and FoxO3a reverse, FoxO3a with TEAD1 and FoxO3a with pcDNA3.1 were analyzed by the Student's t-test, * P < 0.05.