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Figure 6 | BMC Molecular Biology

Figure 6

From: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines

Figure 6

MFAP4 mRNA level is regulated by a unique hUPF1-dependent pathway. A, B. Effects of the knockdown of human NMD factors on MFAP4 mRNA. RNAi was conducted using control siRNA, hUPF1-targeted siRNA (si-hUPF1, in A), or hUPF2-targeted siRNA (si-hUPF2-1, in B) in Jurkat cells. C. Co-immunoprecipitation of MFAP4 mRNA from HPB-ALL cell extracts with anti-hUPF1 antibody. Known NMD targets (gas5 and UHG) and SMD targets (Arf1 and c-jun) were used as positive controls. D, E. Double knockdown of Thy-ncR1 and hUPF1. Immunoblot confirmed the depletion of hUPF1 upon treatment with si-hUPF1 in the presence or absence of si-RNA1 against Thy-ncR1 (D). qRT-PCR of MFAP4, gas5, and Thy-ncR1 in Jurkat cells that were treated with each combination of siRNAs shown

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BMC Molecular Biology

ISSN: 1471-2199

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