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Figure 5 | BMC Molecular Biology

Figure 5

From: A thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator of MFAP4 mRNA in immature T-cell lines

Figure 5

Thy-ncR1 controls MFAP4 mRNA levels. A. Isoform-specific knockdown of Thy-ncR1. The positions of siRNAs designed to knockdown specific Thy-ncR1 isoforms are shown. Isoforms that could be degraded by each siRNA are shown below the arrow. B. Confirmation of Thy-ncR1 knockdown. The levels of Thy-ncR1 isoforms were determined by qRT-PCR. C. Increase in MFAP4 mRNA upon Thy-ncR1 knockdown. The MFAP4 mRNA level in Thy-ncR1 knockdown cells was determined by qRT-PCR. D. RNA hybridization to detect MFAP4 mRNA in HPB-ALL cells treated with control and siRNA 1 of Thy-ncR1.

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BMC Molecular Biology

ISSN: 1471-2199

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