Figure 2

Organization of sequence homologous to Exon 10 of TRα2. A. Overlap between terminal exons of TRα2 and Rev-erbα mRNAs as in Figure 1. Light vertical lines in Rev-erbα coding sequence represent >30 synonymous substitutions within exon 8 of Rev-erbα. Thin horizontal arrows represent C-terminal polypeptides potentially or actually translated from exon 10 of TRα2. Dotted arrow indicates frame-shifted translation product of potoroo and wallaby. Asterisk on longest arrow indicates position of phosphorylation sites on rat TRα2. Double brackets under figure mark sequences highlighted in panels B and C. B. Alignment of 3'ss and 5' end of exon 10. Nucleotide sequence from rat and four marsupials is shown. The amino acid sequence corresponding to exon 10 of TRα2 is shown for the rat and the two opossums. Dashes indicate gaps and dots residues identical to sequence above. Boxes indicate stop codons in-frame with the 3' splice sites for either TRα2 (sense strand) or Rev-erbα (antisense strand). C. Alignment of overlapping coding sequences for TRα2 and Rev-erbα. The amino acid sequence for the rat TRα2 sequence (sense strand) is shown above the nucleotide sequence and the conserved amino acid sequence of Rev-erbα is shown at the bottom. Stop codons are indicated for Rev-erbα (black box, repeated from panel B) and that hypothesized for TRα2 of wallaby and potoroo (white box) assuming use of the homologous 3'ss splice site. The single amino acid substitution in the potoroo Rev-erbα sequence is indicated in italics (A/G). The stop codon for Rev-erbα is shown boxed in both alignments.