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Figure 1 | BMC Molecular Biology

Figure 1

From: Overexpression of miR-128 specifically inhibits the truncated isoform of NTRK3 and upregulates BCL2 in SH-SY5Y neuroblastoma cells

Figure 1

Functional screening of NTRK3 miRNA target sites using a luciferase-based assay. A. Luciferase assay results: FL-NTRK3 and TR-NTRK3 regulation by miRNAs. HeLa cells were cotransfected with pGL4.13-FL or pGL4.13-TR and the indicated miRNA mimics (C = Control). Luciferase activities were measured 24 h after transfection; firefly luciferase activity was normalized to Renilla luciferase activity, and results from at least three independent experiments are presented as means ± SE. Only statistically significant results are shown (p < 0.05). B. The relative location of the target sequences of validated miRNAs in the 3'UTRs of FL-NTRK3 and TR-NTRK3 is shown with an enlargement of overlapping target sites, where sequence pairing to the 3'UTRs (lines) and miRNA seed regions (underlines) are indicated.

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BMC Molecular Biology

ISSN: 1471-2199

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