A mechanism for ramified rolling circle amplification

BMC Molecular Biology

Table 1 Model fit, response time, and analysis data for a real-time RAM reaction.

Targets	10^7	10^7	10^5	10^5	1000	1000	10	10	1	1
exp.eff	1.4	1.3	1.2	1.2	1.3	1.3	1.3	1.4	1.2	1.2
cpD2	15.5	15.2	28.3	30.0	43.8	44.3	54.1	54.9	64.6	69.4
Rt	15	15.4	28.5	30.2	43.8	44	55.6	56	66.6	70.2
R²(adj.)	0.9993	0.9997	0.9996	0.9997	0.9995	0.9999	0.9998	0.9996	0.9997	0.9998

Legend to Table 1.
For each input template level ("Targets") the reaction efficiency (exp.eff., [17]) and second derivative maximum (cpD2) is shown, with the response time (Rt) reported (as Ct) by instrumentation software. cpD2 is the second derivative maxima of signal change vs. time; "R²(adj.)" is the multiple correlation coefficient describing the model's fit to the data.

ISSN: 1471-2199