Western-blot analyses of the functions of miR30-shRNA/nNOS both in vitro (a, b) and in vivo (c, d). a: In vitro tests in PC12 cells. - Dox, cells were cultured in the continuous absence of Dox; + Dox, cells were cultured in the continuous presence of Dox; 1: AD-CMV-nNOS + LVVs-miRnNOS-control1; 2, 3: AD-CMV-nNOS+ LVVs-miRnNOS-neurone; 4: AD-CMV-nNOS+ LVVs-miRnNOS-negative control1; 5: mock transfection. b: In vitro tests in 1321N1 cells. 1: AD-CMV-nNOS + LVVs-miRnNOS-control2; 2, 3:AD-CMV-nNOS + LVVs-miRnNOS-glia; 4: AD-CMV-nNOS + LVVs-miRnNOS-negative control2 5': mock transfection. c, d: In vivo tests in DVC (c) and HIP (d) in rats. Rats in groups 2, 4, were not treated with Dox. Rats in group 3 drunk Dox-containing water post-injection for 7 days. There were 3 rats in each group. 1: mock infection; 2, 3: LVVs-miRnNOS-neurone; 4: LVVs-miRnNOS-negative control2.