Figure 1
From: Cell- and region-specific miR30-based gene knock-down with temporal control in the rat brain
Schematic representation of lentiviral vectors used in the current study. LTR, lentiviral long terminal repeats; Tretight, a modified tetracycline and Dox-responsive promoter derived from pTRE-tight (Clontech); GFP, green fluorescence protein; miR30-shRNA/Luc, miR30-based shRNA targeting firefly Luc gene; miR30-shRNA/nNOS, miR30-based shRNA targeting rat neuronal nitric oxide synthase gene; Luc, firefly Luc gene; GfaABC1D, a compact glial fibrillary acidic protein promoter (690 bp); SYN, human synapsin 1 promoter (470 bp); mCMV, minimal CMV core promoter (65 bp); GAL4BDp65, a chimeric transactivator consisting of a part of the transactivation domain of the murine NF-κBp65 protein fused to the DNA binding domain of GAL4 protein from yeast; WPRE, woodchuck hepatitis post-transcriptional regulatory element.