Table 1 Sequence of Primers and DNA probes

ALCAM Luc reporter constructs

Forward

Reverse

-1200

5'-AAATCACCGCTTAACTCAAAG-3'

5'-CCTCCTCCTTCTTGGTGG-3'

-1000

5'-CCGCGCTTCAACCACCTGCT-3'

-800

5'-CAGAAAGTGTTAGTCCCAGG-3'

-650

5'-CCGCGCTTCAACCACCTGCT-3'

-400

5'-CCGCCTCCTGCGAGTCCTTC-3'

-200

5'-GTTGACCGGGAGGGAGGAGG-3'

EMSA probes

Sense strand

- 850 GATA-1 wt

5'-GCCAGAGGCCTTATCACTGG-3'

- 850 GATA-1 mt

5'-GCCAGAGGCCTGCTCACTGG-3'

ChIP assay

Forward

Reverse

GATA-1

5'-GGGTGGAGGGAGAGGGCAGTC-3'

5'-AAGCAAGCACAGGAGCCGCGC-3'

RT-PCR

Forward

Reverse

ALCAM

5'-AGTCTT CAT TATCAGGATGC-3'

5'-GGGATCAGTTTTCTTTGTCA-3'

γ-globin

5'-ACTCGCTTCTGGAACGTCTGA-3'

5'-GTATCTGGAGGACAGGGCACT-3'

Glycophorin A

5'-GGAATTCCAGCTCATGATCTCAGGATG-3'

5'-TCCACATTTGGTTTGGTGAACAGAT3'

Cmpl

5'-GCACTGTGATGCTTTATGCAAC-3'

5'-TGAACGGTTTAGAGGATGAGGA-3'

CD44

5'-GATCCACCCCAATTCCATCTGTGC-3'

5'-AACCGCGAGAATCAAAGCCAAGGC-3'

CD61

5'-TATAGCATTGGACGGAAGGC-3'

5'-GACCTCATTGTTGAGGCAGG-3'

GAPDH

5'-ATGGGGAAGGTGAAGGTCGG-3'

5'-GACGGTGCCATGGAATTTGC-3'

PKCα

5'-CGAGGAAGGAAACATGGAACTCAG-3'

5'-CCTGTCGGCAAGCATCACCTTT-3'

PKCε

5'-TCAATGGCCTTCTTAAGATCAAAA-3'

5'-CCTGAGAGATCGATGATCACATAC-3'

PKCθ

5'-CTCGTCAAAGAGTATGTCGAATCA-3'

5'-AATTCATTCAGTCCTTTGTGTCACTC-3'

PKCδ

5'-GCATCGCCTTCAACTCCTATGAGCT-3'

5'-ACACACCCACGGTCACCTCAGA-3'