Table 2 PCR primers used in this study.
From: Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages
mRNA | mRNA name | GenBank accession no. | Forward primer1 | Reverse primer1 | Amplicon Size [bp] | PCR efficiency [%] |
|---|---|---|---|---|---|---|
ABL1 | c-abl oncogene 1 | NM_005157.3, NM_007313.2 | GAGCACAGAGACACCACTGACG | GCTCATCTTCATTCAGGCCG | 148 | 100.9 |
ACTB | β-actin | NM_001101.3 | ATTGCCGACAGGATGCAGAA | GCTGATCCACATCTGCTGGAA | 150 | 97.9 |
B2M | β-2-microglobulin | NM_004048.2 | GCTCCGTGGCCTTAGCTGT | ACGTGAGTAAACCTGAATCTTTGGA | 89 | 101.1 |
CD36 | CD36 | NM_001001548.2, NM_001001547.2, NM_000072.3, NM_001127443.1, NM_001127444.1 | TCACTGCGACATGATTAATGGTACA | ACGTCGGATTCAAATACAGCATAGAT | 126 | 99.2 |
EIF2B2 | Eukaryotic translation initiation factor 2B2 | NM_014239.2 | TCCACCCCACTCATCGTCTG | TGGCAGGACTTCTTCAGGAGC | 105 | 101.1 |
G6PD | Glucose-6-phosphate dehydrogenase | NM_000402.3, NM_001042351.1 | CCGTCACCAAGAACATTCACG | GGACAGCCGGTCAGAGCTCT | 107 | 98.7 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_002046.3 | CAACAGCGACACCCACTCCT | CACCCTGTTGCTGTAGCCAAA | 115 | 101.7 |
GUSB | β-glucuronidase | NM_000181.3 | AGCTCATTTGGAATTTTGCCG | GAGTGAAGATCCCCTTTTTATTCCC | 81 | 101.8 |
H3F3A | H3 histone, family 3A | NM_002107.3 | TGGCGCTCCGTGAAATTAGA | TGAGCAATTTCTCGCACCAGA | 91 | 97.0 |
POLR2K | Polymerase (RNA) II (DNA-directed) polypeptide K | NM_005034.3 | ACCCAGAAGGACGTTCAACCTC | TCTCTGCATCTGATTGGATCCC | 107 | 98.9 |
PPARA | Peroxisome proliferator-activated receptor α | NM_001001928.2, NM_005036.4 | AGCCCCTCCTCGGTGACTTAT | GCTTGAGTCGAATCGTTCGC | 172 | 94.6 |
PPARD | Peroxisome proliferator-activated receptor δ | NM_006238.3, NM_177435.2 | AGAACCGCAACAAGTGCCAG | GCATCCGACCAAAACGGA | 87 | 99.1 |
PPARG | Peroxisome proliferator-activated receptor γ | NM_138712.3, NM_015869.4, NM_138711.3, NM_005037.5 | TTCAGAAATGCCTTGCAGTGG | AGCTTCTCCTTCTCGGCCTG | 79 | 100.6 |
PPIB | Peptidylprolyl isomerase B (cyclophilin B) | NM_000942.4 | ATGGCAAGCATGTGGTGTTTG | CCCGGCTGTCTGTCTTGGT | 84 | 96.8 |
PSMB2 | Proteasome subunit, β type 2 | NM_002794.3 | ACGGCAGCAGCTAACTTCACA | TGGCCCTTCATGCTCATCA | 108 | 101.9 |
PSMB6 | Proteasome subunit, β type 6 | NM_002798.1 | GGAATCATCATCGCAGGCTG | CTGCCTTACCATCATACCCCC | 81 | 101.6 |
QARS | Glutaminyl-tRNA synthetase | NM_005051.1 | GAGCGTCTTGGATATTTCTCCGT | GCTTCCAGCTCACACCTTTCC | 108 | 98.3 |
RPL37A | Ribosomal protein L37a | NM_000998.4 | ATTGAAATCAGCCAGCACGC | AGGAACCACAGTGCCAGATCC | 94 | 97.6 |
SRP14 | Signal recognition particle 14 kDa | NM_003134.4 | AGCACTGTGGTGAGCTCCAAG | TCAGCCCATCCATGTTAGCTCTA | 82 | 95.1 |
TUBA1 | α1-tubulin | NM_006009.2, NM_006082.2, NM_032704.3 | GCACTACACCATTGGCAAGGA | AACCAGTTCCCCCACCAAAG | 122 | 98.4 |
TXNRD1 | Thioredoxin reductase 1 | NM_003330.2, NM_182742.1, NM_182729.1, NM_182743.1, NM_001093771.1 | CACAATTGGAATCCACCCTGTC | GCTTGCCCCAGAGCGC | 73 | 99.2 |
UBE2D2 | Ubiquitin-conjugating enzyme E2D 2 | NM_003339.2 | CCAGATGATCCTTTAGTGCCTGAG | ACATCGCATACTTCTGAGTCCATTC | 100 | 102.5 |