Table 1 List of oligonucleotides

Transcription factor^a

OCT1

5'

GGTCTGTGGAAATACTGGCC TAATGGATCCTGAGGTTC

3'

CDX2

5'

GTGGACTGACAGCTTTTCGCC GTCACGTGACACAGTC

3'

USF

5'

GCTTTTTATAGTCAAT TGACACAGTCAAAC

3'

HNF1

5'

CACAGTCAAACATGAG CTTGGTGTATCGA

3'

NF-Y

5'

CTTGGTGTATCGACCT GTTTTTGCCATAT

3'

Electrophoretic mobility shift assays

Putative TFs binding motif in UGT1A1 proximal promoter^a

OCT1

5'

GTGGAAATACTAATT TAATGGA

3'

CDX2

5'

GACTGACAGCTTTTTA TAGTCA

3'

CREB/USF/SREBP1

5'

TTTTTATAGTCACGTG ACACAGTC

3'

HNF1

5'

CACAGTCAAACATTAAC TTG

3'

NF-Y

5'

CTTGGTGTATCGATTGG TTTTTGCCAT

3'

PBX

5'

TGTATCGATT GGTTTTTGCCATATAT

3'

Consensus oligonucleotide probes used in competition assays^a

OCT1 consensus oligo

5'

TGTCGAATGCAAATC ACTAGAA

3'

CDX2 consensus oligo

5'

GTGCAATAAAACTTTA TGAGTA

3'

USF consensus oligo

5'

CACCCGGTCACGTG GCCTACACC

3'

HNF1 consensus oligo

5'

CCAGGTTAATGATTAAC CCA

3'

NF-Y consensus oligo

5'

AGACCGTACGTGATTGG TTAATCTCTT

3'

PBX consensus oligo

5'

CGAATTGATT GATGCACTAATTGCAG

3'

Oligonucleotide probes of the UGT1A1 promoter with CpG sites^b

CpG-4/USF

5'

AGCTTTTTATAGTCACG TGACACAGTCAAACAT

3'

CpG-4/USF-HNF1

5'

TCACG TGACACAGTCAAACATTAACTTGGT

3'

Reverse transcription (RT)-PCR

Target gene

HNF-1α Forward

5'

GGTCATGAGCTTCGTCAACC

3'

HNF-1α Reverse

5'

GCAGGAAGAGATCCGATTCC

3'

USF-1 Forward

5'

GGCAGCTGAGACGGCCTTGG

3'

USF-1 Reverse

5'

TGGCCCCCATTCTCAGTTCGGA

3'

USF-2 Forward

5'

CTGCCTCTGTGCCCCCAGGT

3'

USF-2 Reverse

5'

TTGTCCCTCCGCCTCCGCTC

3'

GAPDH Forward

5'

TTGGTATCGTGGAAGGACTCA

3'

GAPDH Reverse

5'

TGTCATCATATTTGGCAGGTTT

3'