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Figure 6 | BMC Molecular Biology

Figure 6

From: Regulation of UGT1A1 and HNF1 transcription factor gene expression by DNA methylation in colon cancer cells

Figure 6

Upregulation of HNF1A expression is observed following treatment with the 5-Aza-dC demethylating agent in UGT1A1-negative HCT116 colon cancer cells. Rescue of HNF1A gene expression by 5-Aza-dC treatment of colon carcinoma cell lines. Total RNA from HT29 and HCT116 cells were randomly converted to cDNA and specific amplification for USF1, USF2, and HNF1 mRNAs were carried out. Expression of USF1 and USF2 mRNAs are not influenced by 5-Aza-dC treatment. GAPDH gene is used as internal control. Untreated; cells not exposed to 5-Aza-dC.

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