Localization of PML II to the nuclear periphery. A) Immunofluorescence showing FLAG-tagged PML I, II and III in lentivirus transduced U2OS cells. Overexpressed FLAG-PML II localizes to the nuclear periphery and causes exclusion of lamina. B) Relocalization of endogenous PML to the nuclear periphery in DRAQ5 or Actinomycin D treated cells. Cells were treated with DRAQ5 (2 μM) or Actinomycin D (5 μg/ml) for 2 and 4 hours respectively before fixation. Quantitation of results was performed by scoring cells with detectable PML at the nuclear periphery. The data represent the average of two independent experiments ± standard deviation. The number of cells containing rearranged PML is significantly increased in drug treated versus control treated cells (p < 0.005).