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Table 1 Plasmids used in this study

From: Translational independence between overlapping genes for a restriction endonuclease and its transcriptional regulator

Name

Relevant Feature(s)

Reference

pDK200

WT pvuIIC gene with its own promoter, ΔpvuIIM,

[7]

pDK201

ΔpvuIIR; TetR, pACYC177

[7]

pDK201-Esp33

pDK200 with pvuIIC- Esp19 in-frame insertion

This study

pDK201-Cla35

pDK200 with pvuIIC- Esp33 in-frame insertion

This study

pPvuIIRM3.4

pDK200 with pvuIIC- Cla35 frameshift mutation

[12]

pPvuIIRM3.4- Esp19

WT PvuII R-M system; AmpR, pBR322

[17]

pLex3B

pPvuIIRM3.4 with pvuIIC- Esp19 in-frame insertion

[58]

 

Plasmid from ATCC (#87200) used to create translational fusions, to lacZ; AmpR, pMB1 origin

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