Figure 5

Stop codons created in pvuIIC upstream of pvuIIR-lac Z fusion initiation site. The native pvuIIC translation terminator is downstream of the pvuIIR initiation codon (see top line of Figure 2). A. To test for translational coupling, site-directed mutation was used to introduce pvuIIC terminators farther upstream. The pvuIIR RBS and intiation codon are indicated in green. The two introduced stop codons were in two independent clones. B. lacZ activity of the WT (filled circles) and the mutants (open squares have terminator indicated "S1" in part A; filled squares represent S2). The linear fit is to the WT data.