Germline transformation of the stalk-eyed fly, Teleopsis dalmanni

BMC Molecular Biology

Table 4 Segregation analysis for transgenic line 34.

Fly	Sex	G2 larvae		Sex of surviving G2 offspring
		EGFP positive	EGFP negative	EGFP positive		EGFP negative
				Male	Female	Male	Female
34.3	♀	12	20	3	3	1	2
34.4	♀	52	74	22	20	16	15
34.5	♂	12	33^§	1	6	10	10
34.6	♂	50	71	13	17	22	16
34.7	♂	65	172^§	0*	32	70	38
34.8	♀	24	47^§	7	13	8	15
34.9	♀	7	11	5	2	1	2
34.10	♀	15	103^§	4	6	26	35
34.11	♂	30	100^§	0*	14	27	6
34.14	♂	96	215^§	0*	72	41	17
34.15	♂	10	43^§	0*	5	5	6

Surviving fertile EGFP positive G1 progeny of the G0 individual, fly 34, were individually crossed with wild-type virgin flies of the opposite sex to generate G2 offspring. G2 offspring were screened for EGFP fluorescence during the third larval instar and sex was recorded when individuals eclosed. Not all G2 progeny were reared to adulthood. A single X-linked insertion in a male G1 carrier would lead to all female offspring being EGFP positive and all males being EGFP negative. Autosomal insertions would be inherited by both female and male G2 offspring. *In four male derived sublines there was a significant sex bias among EGFP positive offspring with EGFP positive males being absent. Both male and and female EGFP positive offspring were produced by males 34.5 and 34.6.
§There was a significant deficit of EGFP positive offspring in seven sublines.

ISSN: 1471-2199