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Table 2 Transformation frequencies for three injection experiments.

From: Germline transformation of the stalk-eyed fly, Teleopsis dalmanni

Experiment

Number of eggs injected

Number of G0 adults

Number of fertile G0 adults

Number of G1 individuals screened

Number of G0 transformants

Transformation efficiency (%)

Transformationrate (%)

1

135

6

2

323

1

50

0.74

2

123

5

3

34

0

0

0

3

441

63

45

4934

1

2.2

0.23

TOTAL

699

74

50

5291

2

4

0.28

  1. Transformation efficiency is given as the percentage of fertile G0 adults giving rise to transgene-expressing G1 offspring (G0 transformants). Transformation rate is given as the percentage of injected eggs that gave rise to transformants.