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Figure 3 | BMC Molecular Biology

Figure 3

From: Germline transformation of the stalk-eyed fly, Teleopsis dalmanni

Figure 3

Molecular characterisation of insertions. (A). Southern analysis confirms transgene integration. DNA was extracted from individuals from four G1 individual derived lines carrying the transgene (17.1, 17.2, 34.6 & 34.14) and digested with Eco RI. The probe was generated from the Not I/Sal I fragment of pMi[3xP3-EGFP]. DNA from wild-type (WT) individuals was included as a control. For lines 17.1 and 17.2 single bands of the same size (approximately 6500 bp) are present. For lines 34.6 and 34.14 the multiple bands are indicative of multiple inserts at different locations in the genome. No bands are present in wild-type DNA. (B). Flanking sequence of right arm of an insert present in subline 34.6. The pMi[3xP3-EGFP] sequence is shown in upper case and the flanking genomic sequence in lower case. TA duplication, characteristic of a Minos mediated insertion event, is underlined.

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