Figure 2

EGFP expression in transgenic larvae. Larvae are from line 34.6 (A, B), line 17.1 (D,E) or non-transgenic (C). In both transgenic lines EGFP fluorescence was restricted to the posterior-most segments of the larva. Asterisks denote anal pads and ps denotes posterior spiracles. (A) Posterior end of a first instar larva showing EGFP expression in the anal pads. Note the position of the posterior spiracles. (B) Posterior end of a third instar larva showing strong EGFP fluorescence in mature anal pads. (C) Posterior end of a non-transgenic third instar larva. A degree of autofluorescence is visible in the cuticle but not in the anal pads. (D) Full length third instar larva showing EGFP expression at the posterior end. Autofluorescence is visible in the gut. (E) High power view of anal pad EGFP expression of the larva shown in (D). Scale bar in A = 100 μm; in B, C, and E = 200 μm; in D = 1 mm.