Figure 6
From: A tryptophan-rich peptide acts as a transcription activation domain
Assays of the transcriptional activity of W7using a two-hybrid system. (A) Two-hybrid assays. W7 and (WYDWW)2 were expressed as fusions to the SV40 large T-antigen, and the abilities of the resulting fusions to specifically interact with Gal4-DBD-53 and turn on the reporter genes (HIS3 and MEL1) were tested. For clarity, the bait and prey fusion proteins encoded by the constructs are depicted in parentheses following the vector names. (B) Quantitative assays of β-gal activity. The numbers (circled) in A and B represent the constructs shown in A.