Figure 3
From: A tryptophan-rich peptide acts as a transcription activation domain
Assays of the transcriptional activities of native and mutant pentapeptides. (A) Transcriptional assays. Native and mutant pentapeptides were expressed as fusions to the specific DNA-binding protein, LexA, and the abilities of the resulting fusion proteins to turn on the reporter genes (LEU2 and lacZ), which are controlled by distinct LexA-responsive promoters, were tested. (B) Quantitative assays of β-gal activity. (C) Western blot analysis of the expressions of LexA fusion proteins. Upper panel, LexA fusion protein; lower panel, phosphoglycerate kinase (PGK) (as a loading control). The numbers (circled) in A, B, and C represent the constructs shown in A.