Figure 3

Quantitative luciferase analysis of αDENV-GrpIs splicing efficiency. A) Schematic diagram of the αDENV-GrpI-FL constructs used for this analysis. A5c = Drosophila melanogaster actin 5c promoter, FL = firefly luciferase. B) Drosophila S2 cells were co-transfected with 3 μg αDENV-GrpIs, 0.5 μg of the IRL expression plasmid, and 1 μg of either double-stranded DENV-2 target (+) or control plasmid, pUC57 (-) (see Methods). Following cell lysis, samples were processed using the Dual Luciferase System (Promega) according to the manufacturer's direction. The error bars represent standard deviations of three independent experiments.