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Figure 2 | BMC Molecular Biology

Figure 2

From: Identification of amino acid residues in protein SRP72 required for binding to a kinked 5e motif of the human signal recognition particle RNA

Figure 2

5e RNA binding activities of polypeptides 72 e, 72 f, 72 g, 72 h and 72 j. Purified his-tagged polypeptides 72 e to 72 j were incubated with in vitro transcribed 5e SRP RNA and Ni-NTA magnetic agarose beads as described in the Methods. The bound protein and RNA were analyzed by SDS PAGE followed by staining of the same gel with Coomassie blue (lanes labeled p) and Ethidium bromide (lanes labeled r). Molecular mass markers in kDa are shown in lane m. Plus signs indicate the formation of complexes, the minus sign below 72 g indicates that this polypeptide was unable to bind. Variable amounts of a material which probably represented 5e SRP RNA dimers were observed (arrow heads).

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