Figure 7
From: Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol
Precipitation by ethanol of cDNA from single GH4 cells reduced the mean Cq value. The grey dots in each chart representing individual Cq values for each cell- or cytosol-harvest with black lines representing mean ± SD. First chart column. Initially, qPCR on single cells was performed directly following reverse transcription, without any purification of cDNA. This gave a mean Cq value of 35.99 ± 2.82 (n = 15). Second chart column. After ethanol precipitation, the mean Cq value was reduced to 31.23 ± 0.88 (n = 9), achieving a significantly reduced mean Cq and significantly difference in variance (F-test with P = 0.0013). Third chart column. Harvesting of cytosol revealed no difference in mean Cq 30.87 ± 1.29 (n = 11) compared to harvesting of whole cells. The variance was similar (F-test with P = 0.14) when comparing the two harvesting techniques.