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Figure 6 | BMC Molecular Biology

Figure 6

From: Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol

Figure 6

Sensitivity of the follicle-stimulating hormone beta subunit ( FSHβ )-, and luteinizing hormone beta subunit ( LHβ ) - primer pairs as measured in copy numbers. By first amplifying the complete coding region of FSHβ and LHβ, we made a cDNA stock solution with defined molecule numbers. From the stock, a series of dilutions in triplicate with five- and two-fold increments was made starting from 100 000 molecules. Linearity for both the FSHβ (A) and LHβ (B) primer pairs were shown down to 3 molecules. The detection limit for the FSHβ primer pair was 1 copy (C), and for the LHβ primer pair it was 3 copies (D).

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